2 g
Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Research on Microbes
Microbiology Experts
Online Submission
Access Statistics

Site Visitors

blog tracking

Preparative Biochemistry and Biotechnology
Vol. 45 (5), 2015, Pages: 491-499

Biodegradation of Isopropanol by a Solvent-Tolerant Paracoccus denitrificans Strain

Yucong Geng, Yuanjie Deng, Feilong Chen, Hong Jin, Ke Tao & Taiping Hou

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education , College of Life Sciences, Sichuan University , Chengdu , P.R. China.


The biodegradation of high concentration isopropanol (2-propanol, IPA) at 16 g/L was investigated by a solvent-tolerant strain of bacteria identified as Paracoccus denitrificans for the first time by 16S rDNA gene sequencing. The strain P. denitrificans GH3 was able to utilize the high concentration of IPA as the sole carbon source within a minimal salts medium with a cell density of 1.5 × 108 cells/mL. The optimal conditions were found as follows: initial pH 7.0, incubation temperature 30°C, with IPA concentration 8 g/L. Under the optimal conditions, strain GH3 utilized 90.3% of IPA in 7 days. Acetone, the major intermediate of aerobic IPA biodegradation, was also monitored as an indicator of microbial IPA utilization. Both IPA and acetone were completely removed from the medium following 216 hr and 240 hr, respectively. The growth of strain GH3 on IPA as a sole carbon and energy source was well described by the Andrews model with a maximum growth rate (μ max ) = 0.0277/hr, a saturation constant (KS ) = 0.7333 g/L, and an inhibition concentration (Ki) = 8.9887 g/L. Paracoccus denitrificans GH3 is considered to be well used in degrading IPA in wastewater.

Keywords: acetone, Andrews model, biodegradation, isopropanol (IPA), Paracoccus denitrificans, specific growth rate.

Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution