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Methods in Enzymology
Volume 630, 2020, Pages 133-158

Improvement in biochemical characteristics of cross-linked enzyme aggregates (CLEAs) with magnetic nanoparticles as support matrix

Nithyakalyani Doraiswamya, Mahalakshmi Sarathia, Gautam Pennathura,b

Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India.


Recent developments in novel carriers for enzyme immobilization have led to improvement in the stability and cost-effectiveness of the biocatalysts for their enhanced suitability in the industrial applications. Cross-linked enzyme aggregates (CLEAs), a recent technique developed in the carrier-free type of enzyme immobilization is a simple and straightforward method. Moreover, the magnetic property and the higher surface-to-volume ratio of the maghemite nanoparticles have also been utilized in the present immobilization technique as magnetic nanoparticle-supported CLEAs (Mgnp-CLEAs). The stability studies of the free and immobilized enzyme revealed the Mgnp-CLEAs to have enhanced enzyme stability with an increase in the reusability cycle. The physical characterization of the nanoparticles and immobilized enzymes by the Scanning Electron Microscopy (SEM), Fourier-Transform Infrared spectroscopy (FT-IR) and X-ray diffraction analysis (XRD) showed the successful immobilization of the enzyme for its improved stability.

Keywords: Immobilization, Hydrolytic enzymes, Magnetite nanoparticle, CLEAs, Fourier-Transform Infrared Spectroscopy, X-ray diffraction.

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