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Journal of Pharmaceutical and Biomedical Analysis
Vol. 113, 2015, Pages: 181–188

Toward greener analytical techniques for the absolute quantification of peptides in pharmaceutical and biological samples

Ann Van Eeckhaut, Debby Mangelings

Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.


Peptide-based biopharmaceuticals represent one of the fastest growing classes of new drug molecules. New reaction types included in the synthesis strategies to reduce the rapid metabolism of peptides, along with the availability of new formulation and delivery technologies, resulted in an increased marketing of peptide drug products. In this regard, the development of analytical methods for quantification of peptides in pharmaceutical and biological samples is of utmost importance. From the sample preparation step to their analysis by means of chromatographic or electrophoretic methods, many difficulties should be tackled to analyze them. Recent developments in analytical techniques emphasize more and more on the use of green analytical techniques. This review will discuss the progresses in and challenges observed during green analytical method development for the quantification of peptides in pharmaceutical and biological samples.

Graphical abstract

Keywords: Peptide; Green; Liquid chromatography; Capillary electrophoresis; Supercritical fluid chromatography.

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