8 6 5 2 1 2 g
Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
Access Statistics

Site Visitors

blog tracking


 
Process Biochemistry
Vol. 51 (8), 2016, Pages: 1028–1039

Unanticipated behaviour of sorbitol towards the stability and activity of stem bromelain: An outlook through biophysical techniques

Anjeeta Rani, Venkatesu Pannuru

Department of Chemistry, University of Delhi, Delhi, 110 007, India.

Abstract

As far as the increased risk of the diseases attributed to the misfolded/unfolded/aggregated proteins are concerned, one may not deny the importance of the various methods (chemical modifications of enzyme, protein engineering techniques and additives) which maintain the protein in its three dimensional functional native form. Out of these methods, the interest in the additives has been grown rapidly in last few decades. Among these additives, polyols are well known compatible co-solvents for the protein. We have investigated the influences of a series of structurally related polyols (ethylene glycol, glycerol, erythritol, xylitol and sorbitol) on the stability and activity of stem bromelain (BM) by using fluorescence spectroscopy, circular dichroism (CD), UV–vis, Fourier transform infrared (FTIR) spectroscopy and dynamic light scattering (DLS). Our results revealed the increased conformational stability of BM with increase in the size of polyols and the proteolytic activity of BM in reverse order. However, sorbitol was found to be the weakest stabilizer for BM among all polyols whereas BM possessed highest activity in the same. To the best of our knowledge, our results represent first detailed unambiguous proof of unusual effect of sorbitol on the interactions governing stability of BM.

Graphical abstract

Keywords: Bromelain; Polyols; Activity; Protein folding; Biophysical techniques.

Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution