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International Journal of Hydrogen Energy
Vol. 41 (26), 20
16, Pages: 11072–11080

Microbial dynamics of the extreme-thermophilic (70C) mixed culture for hydrogen production in a chemostat

Fang Zhang, Jing-Hua Yang, Kun Dai, Zhao-Wei Ding, Long-Gang Wang, Qiu-Rong Li, Fa-Ming Gao, Raymond J. Zeng

Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei, 066004, People's Republic of China.


Most studies of mixed culture fermentation for hydrogen production focus on the metabolite distribution under different operational conditions. While, the corresponding microbial dynamics research is limited. The aim of this work was to reveal the microbial community dynamics of extreme-thermophilic mixed culture enriched in a chemostat for hydrogen production with the combining molecular tools of denaturing gradient gel electrophoresis (DGGE), Illumina Miseq high-throughput sequencing platform, and 16S rRNA clone library sequencing. The analyzed results showed that the genera ofCaldanaerobius, Caldicellulosiruptor and/or Thermoanaerobacter dominated the bacteria. While archaea were not detected even by Illumina Miseq high-throughput sequencing. The effects of hydrogen partial pressure or pH increasing from 5.5 to 7.0 on the microbial communities were not notably, and genera of Caldicellulosiruptor andThermoanaerobacter were the dominants. High hydrogen yield in this chemostat was attributed to the enrichment of Caldicellulosiruptor. While Caldanaerobius dominated the bacteria communities as pH decreased to 4.5. Caldanaerobius and Thermoanaerobacterdominated at high influent glucose concentrations. Thereby, it clearly indicated that the microbial dynamics shall be considered when analyzing the extreme-thermophilic mixed culture fermentation in the future.

Keywords: Mixed culture fermentation; Extreme-thermophilic; Microbial dynamics; DGGE; Illumina Miseq sequencing; 16S rRNA clone library.

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