Recent advances in microbiology have revealed that bacteria are able to communicate and cooperate in a wide range of multicellular behaviors such as dispersal, foraging and biofilm formation, in a process called quorum sensing (QS). In the QS-regulated communication, bacteria produce and secrete small signaling molecules – autoinducers that are recognised by specific receptors. Gram-negative bacteria secrete acyl-homoserine lactones (AHLs) that in threshold concentrations penetrate into the cells and activate cognate intracellular AHL receptors, while gram-positive bacteria produce autoinducing peptides that are detected by the cell membrane histidine kinase receptor. However, not all bacterial communication is species specific. Bacteria also possess a receptor for the signals sent out by other bacteria species. The knowledge about how this intra- and inter-species communication occurs has been increasingly used to develop new strategies for fighting infectious diseases. This chapter summarises recent advances for silencing cell-to-cell communication as a new approach for the prevention of bacterial diseases.