Biodegradation
as a Remedial
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"Naturally
occurring biodegradation" means degradation
of organic compounds by indigenous microbes without
artificial enhancement. The terms "passive
bioremediation" and "intrinsic bioremediation"
are also used to describe utilization of naturally
occurring biodegradation as a remedial action.
Application of naturally occurring biodegradation
as a remediation technique requires that a site
be evaluated to ensure site conditions are appropriate
and that a monitoring plan be developed.
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Bioremediation
Application
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Bioremediation
is a treatment process that uses naturally occurring
microorganisms (yeast, fungi, or bacteria) to
break down, or degrade, hazardous substances into
less toxic or nontoxic substances. Microorganisms,
just like humans, eat and digest organic substances
for nutrients and energy. In chemical terms, “organic”
compounds are those that contain carbon and hydrogen
atoms. Certain microorganisms can digest organic
substances such as fuels or solvents that are
hazardous to humans.
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Microbial
genomes from the environment
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Microbial
communities are vital in the functioning of all
ecosystems; however, most microorganisms are uncultivated,
and their roles in natural systems are unclear.
Here, using random shotgun sequencing of DNA from
a natural acidophilic biofilm, we report reconstruction
of near-complete genomes of Leptospirillum group
II and Ferroplasma type II, and partial recovery
of three other genomes. This was possible because
the biofilm was dominated by a small number of
species populations and the frequency of genomic
rearrangements and gene insertions or deletions
was relatively low. Because each sequence read
came from a different individual, we could determine
that single-nucleotide polymorphisms are the predominant
form of heterogeneity at the strain level. |
Transgenic
Plants and Microbes
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The
use of biotechnology to modify plants has become
a common practice in agricultural and horticultural
research. Unlike ordinary research materials used
in laboratory, greenhouse, and field studies,
transgenic (genetically engineered, genetically
modified)1 organisms are subject to special rules
intended to ensure that they are used in a way
that does not pose an unacceptable risk to human
health or the environment. |
Microbial
Enzymes and Their Applications
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Enzymes
are biocatalysts produced by living cells to bring
about specific biochemical reactions generally
forming parts of the metabolic processes of the
cells. Enzymes are highly specific in their action
on substrates and often many different enzymes
are required to bring about, by concerted action,
the sequence of metabolic reactions performed
by the living cell. All enzymes which have been
purified are protein in nature, and may or may
not possess a nonprotein prosthetic group. |
Naturally
Occurring Biodegradation
|
"Naturally
occurring biodegradation" means degradation
of organic compounds by indigenous microbes without
artificial enhancement. The terms "passive
bioremediation" and "intrinsic bioremediation"
are also used to describe utilization of naturally
occurring biodegradation as a remedial action.
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Cleaning
up environmental pollution
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Some pollutants,
such as pesticides or fertilizers, are used to
benefit agriculture and enter the environment
intentionally. Others, such as solvents, are toxic
by-products of industrial processes that yield
medicines or other products for which there is
consumer demand.
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Bioremediation:
Cost-effective Alternative to Clean Environmental
Messes
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Enormous quantities
of organic and inorganic compounds are released
into the environment each year as a result of
human activities. In some cases, these releases
are deliberate and well regulated (e.g., industrial
emissions) while in other cases they are accidental
(e.g., chemical or oil spills). Many of these
compounds are both toxic and persistent in terrestrial
and aquatic environments.
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MICROBES
AND GLOBAL ENVIRONMENTAL CHANGE |
Despite the
diminutive size of individual microbial cells,
the high abundance of microorganisms in virtually
all habitats ensures that they contribute significantly
to global biomass. It has been estimated recently
that the carbon biomass of prokaryotic microbes
is 60-100% of that thought to occur in plants
worldwide, and microbial nitrogen and phosphorus
biomass exceeds that of all other organisms.
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MICROBIAL
ECOLOGY - ITS FUNDAMENTAL ROLE IN SUSTAINABLE
AQUACULTURE |
For intensive
aquaculture to become a sustainable industry with
minimal environmental impact microbial ecology
is critically important as a scientific discipline;
applications of microbial ecology are needed at
the forefront of advances now being made. Water
quality and the control of disease are interdependent
and linked to the microbial, especially bacterial,
activities in ponds. |
New
Approaches for Bioaugmentation as a Remediation
Technology |
Bioaugmentation
is commonly employed as a remediation technology.
However, numerous studies indicate that introduced
microorganisms often do not survive in the environment
and thus do not increase contaminant remediation.
This review details several new approaches that
may increase the persistence and activity of exogenous
microorganisms and/or genes following introduction
into the environment. These techniques include:
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Bioremediation
of Sewage Sludge by Compositing |
Sewage sludge used as soil organic amendment
can positively affect soil physical, chemical,
and biological properties. This organic material
may be a solution for improving the low organic
matter content of Mediterranean soils. However,
the addition of sewage sludge to soils have
not always shown positive effects, probably
due to the particular characteristics of the
residues concerned: high heavy metal or phytotoxic
substance content, excess of labile organic
matter, pathogenic microorganisms, etc:
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Effects
of Crude Oil, Oil Components, and Bioremediation
on Plant Growth |
The phytotoxic
effects of crude oil and oil components on the
growth of red beans (Phaseolus nipponesis OWH1)
and corn (Zea mays) was investigated. In addition,
the beneficial effects of bioremediation with
the oil-degrading microorganism, Nocardia sp.
H17-1, on corn and red bean growth in oil-contaminated
soil was also determined. It was found that crude
oil-contaminated soil (10,000 mg/kg) was phytotoxic
to corn and red beans.
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Multi-Species
Ecotoxicity Assessment of Petroleum-Contaminated
Soil |
In 1992, a
study was begun to compare the effect of landfarming
vs. natural attenuation on the restoration of
soil that had been contaminated with crude oil.
Each of three lysimeters was filled with a sandy
loam topsoil, and crude oil was applied to two
of the lysimeters. One of the contaminated lysimeters
was tilled, watered, and received a one-time application
of fertilizer (N, P, K). No amendments were added
to the second contaminated lysimeter, and the
third was left uncontaminated. The lysimeters
were monitored for 6 months and then left unattended.
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Biodegradation
of Natural Oils in Seawater |
Spills of non-petroleum
hydrocarbons including vegetable oils and fish
oils are of environmental concern because of their
potential to cause serious effects on marine life
and coastal environments. Biodegradation by indigenous
microorganisms is an important and potentially
ubiquitous process affecting both the chemical
composition and physical properties of contaminant
oils. Data on the environmental persistence of
non-petroleum oils is now required for risk assessments
and decision making by spill responders. This
article investigates the biodegradability of various
vegetable and fishoils under the influence of
natural bacteria in seawater.
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Properties
and Classification of Microorganisms |
Just what are
microorganisms? They are small living forms of
life, which we cannot see with the naked eye.
Bacteria, yeasts, and molds are three types of
microorganisms. Some people often confuse, and
almost always misunderstand, their functions,
but they are just as real and alive as you are.
They eat and grow. They reproduce and die. Have
you ever wondered just how small microorganisms
really are? Molds can be seen with only slight
magnification and use of an ordinary magnifying
glass. Yeasts must be viewed through a microscope
that magnifies several hundred times. Bacteria
can best be seen when studied with a more powerful
microscope that enlarges 1,000 times.
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Classification
of Microorganism Taxonomy |
By carbon and
energy source, By cell structure, By genetic sequencing,
By cell shape - morphology, By gram stain, By
how they grow, By O2 requirements as electron
acceptor, Other Classification Methods, Cell Chemistry,
Parts of the Procaryotic Microbial Cell, Chemical
Thermodynamics Review, Measurement of Organic
Carbon, Microbial Metabolism, Respiration, Microbial
Metabolism Review, Alternate Modes of Energy Metabolism,
Measurement of Microbial Biomass, Genetics and
Information Flow, Microbial Growth, Continuous
growth of microbial cultures, CFSTR with Biomass
Recycle: Activated Sludge.
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Uptake
of Heavy Metals by Microorganisms |
Contamination
in drinking water is the most common form of environmental
problems encountered in water resources management.
Some contaminants, present accidentally in drinking
water, are very difficult to remove, such as heavy
elements that are products of industrial waste.
Lead is one of the most difficult-to-remove elements.
This paper proposes a novel process for removal
of lead compounds contaminants from water. The
proposed method shows great efficiency.
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Bacteria
for Improvement of Oil Recovery |
In microbial
enhanced oil recovery (MEOR) technique, microorganisms
and/or their products (gases, chemicals) are used
in the enhancement of oil recovery. In the present
study, MEOR is tested for Garzan (26° API)
crude oil, produced from Southeast Turkey. This
work consists of shut-in pressure tests and microbial
water ooding experiments. In shut-in pressure
tests, the oil is placed in a stainless steel
cell and a certain amount of microbial solution
(Clostridium acetobutylicum) is introduced. During
the soaking period, the pressure increase is monitored. |
Anaerobic/aerobic
treatment of colorants present in textile effluents |
The operation
of an anaerobic/aerobic process used to degrade
the colorants present in textile wastewater is
presented. The objective is to produce water that
can be reused. Two particular cases were studied:
the degradation of a synthetic wastewater containing
the colorant disperse blue 79 (DB79) as a model
compound and a real textile effluent containing
reactive azo dyes. The biodegradation was achieved
using a single tank operated as sequencing batch
reactor. It was observed that the DB79 was biotransformed
to amines in the anaerobic stage decolorizing
the wastewater.
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Natural
clean-up for oil-spill soils |
Oil spills
from tankers or simply your local garage could
soon be cleaned up using specially-selected bacteria,
according to research presented today (Wednesday,
06 April 2005) at the Society for General Microbiology's
156th Meeting at Heriot-Watt University, Edinburgh.
Millions of gallons of crude oil and its derivates
used by the plastics and chemical industries are
transported vast distances around the world every
day, and inevitably some of it gets spilled. Scientists
from University College Dublin are studying how
natural bacteria can be used to tackle these pollutants.
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Global warming
plus natural bacteria could release vast carbon
deposits
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Increasing concentrations of carbon dioxide in
the atmosphere will make global temperatures rise.
By studying soil cores from the Arctic, scientists
have discovered that this rise in temperature
stimulates the growth of microorganisms that can
break down long-term stores of carbon, releasing
them into the atmosphere as carbon dioxide. This
will lead to further increases in global temperatures.
Carbon is held in soil either in material that
is easily degraded by chemical and bacterial action
(labile soil carbon), or in material that is less
easily degraded by microorganisms (resistant soil
carbon). About one third of the world's soil carbon
is located in high latitudes such as the Arctic,
and much of this effectively locked away in recalcitrant
stores.
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Home
composting and its role in waste management |
Composting
is the aerobic microbial degradation of bulky
organic waste, which usually generates heat, to
produce a stabilized residue with significant
value as a soil conditioner. The advantages of
this natural process for treating biodegradable
waste have placed it in a priority role for delivering
the Government's target reductions in household
waste disposal to landfill. Many householders
with horticultural interests have traditionally
composted and re-used their garden waste (Fig.
1). Encouraging and developing participation in
home composting schemes also has major potential
advantages in providing a low-cost approach to
waste management and facilitating the sustainable
recycling of biodegradable organic waste (Fig.
2).
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Bugs
within bugs: symbiotic bacteria in garden insects |
Macrobiologists would give a microbefree answer
to this question. The animals consuming garden
plants - mostly insects - are kept in check by
a combination of plant defences which make the
plants unpalatable or downright toxic, and natural
enemies such as ladybirds and other predatory
beetles, parasitic wasps and fungal pathogens.
This approach neglects a key weapon of phytophagous
(plantfeeding) insects in their fight back against
the near-inedible plants and army of natural enemies:
symbiotic micro-organisms. Many of the microbes
requisitioned by phytophagous insects in their
unending war with plants and natural enemies cannot
be cultured by traditional laboratory methods.
However, the recent development of molecular techniques
to study unculturable forms is permitting many
new discoveries to be made about these micro-organisms.
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Soil
microbes and the war on garden weeds |
Weeds have
been associated with human activity from the very
beginnings of society, when man evolved from a
hunter-gatherer mindset and began to domesticate
plants and animals and cultivate the land on which
he settled. Why have weeds continued to persist
as problems in our gardens, lawns, fields, meadows,
and roadsides to this day? What is the nature
of weeds that causes them to be opportunistic
in almost any environmental situation and pose
problems ranging from competition with man's desirable
plants, to production of allergens, production
of complex chemicals often toxic to man and animal,
contamination of foods, feed and fibre, and harbouring
other pests? In today's society it seems to be
easier to find a 'quick fix' for problems rather
than try to understand why the problem exists
and derive a management plan based on knowledge.
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Bacterial
and fungal diseases of garden plants |
The main reason
is that seedlings are fleshy and juicy whereas
seeds contain reserves of carbohydrates and other
stored food reserves used in germination. Many
seedlings are either killed or incapacitated by
a mixed group of micro-organisms which cause damping-off
and foot rots. The protozoan oomycete 'pseudofungi',
in the genus Pythium, are often the chief culprits,
causing damping off. The disease is worse in damp
or wet soils. The closely related oomycete Phytophthora
spp. behave similarly, but also attack the fleshy
newly emerged roots and stem collars on much more
mature plants, including a number of trees. |
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